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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
JoVE Journal
Biologia
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JoVE Journal Biologia
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

DOI:
10.3791/51728-v

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10:32 min

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September 06, 2014

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, , , ,
1Department of Anatomy and Developmental Biology,Monash University, 2Australian Regenerative Medicine Institute,Monash University

Capítulos

  • 00:05Título
  • 01:19Generation of Mouse Embryonic Fibroblasts (MEFs)
  • 03:17Reprogramming of MEFs
  • 05:14Antibody Labeling
  • 06:50FACS Isolation of Intermediates
  • 08:26Results: Representative Images and FACS Profiles of Reprogramming MEF Cultures
  • 10:09Conclusion

Summary

Tadução automática

Tadução automática

Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.

Tags

Keywords: Cell Surface MarkerIPS CellReprogrammable Mouse ModelInduced Pluripotent Stem CellsReprogrammingCell Surface Marker ExpressionThy-1.2Ssea-1EpcamMouse Embryonic FibroblastsOKSMDoxycyclineFluorescent Activated Cell Sorting

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