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Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
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JoVE Journal Bioengenharia
Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars

DOI:
10.3791/51732-v

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07:19 min

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August 20, 2014

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, , , , , ,
1Department of Chemical and Material Engineering,University of Alberta, 2Department of Civil and Environmental Engineering,University of Alberta, 3Department of Mechanical Engineering,Texas A&M University, 4Department of Mechanical Engineering,University of Alberta

Capítulos

  • 00:05Título
  • 00:51Microfluidic Chip Fabrication: PDMS Casting
  • 02:39Bacterial Culture
  • 04:01Biofilm Generation Experiment
  • 05:21Streamer and Biofilm Formation by Pseudomonas fluorescens
  • 06:45Conclusion

Summary

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Tadução automática

Protocols for the study of biofilm formation in a microfluidic device that mimics porous media are discussed. The microfluidic device consists of an array of micro-pillars and biofilm formation by Pseudomonas fluorescens in this device is investigated.

Tags

Keywords: BiofilmMicrofluidic DeviceMicro-pillarsPseudomonas FluorescensPorous MediaStreamer FormationSoft LithographyBacterial CultureFlow Experiments

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