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作为一种工具来描绘分子途径参与神经毒性和神经保护神经元细胞在线实时阻抗为基础的细胞分析仪
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
DOI:

08:23 min

August 09, 2014

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Capítulos

  • 00:05Título
  • 01:39Plating and Proliferation of SK-N-SH Cells
  • 03:59Serum Deprivation
  • 05:04Data Analysis
  • 06:11Results: Representative Neurotoxicity/Neuroprotection Data
  • 07:53Conclusion

Summary

Tadução automática

Improved in vitro neurotoxicity assays would aid the identification of new neuroprotective compounds. The utility of a real-time impedance-based cell analyzer to determine cytotoxicity and cytoprotection in neuronal cell lines and to delineate the involvement of second messenger pathways, thus gaining insight in the mechanism of neuroprotection is presented.

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