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Stiksår Skade af zebrafisk Adult telencephalon: En metode til at undersøge Vertebrate Brain Neurogenese og Regeneration
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Capítulos
- 00:05Título
- 01:32Generating a Stab Wound in the Telencephalon
- 02:21Analyzing the Effect of Telencephalic Injury
- 04:36Trimming the Agarose Block and Sectioning the Brain
- 06:36Immunohistochemistry on Vibratome Sections
- 07:28Telencephalon Stab Wound Results
- 08:49Conclusion
To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.
