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分离，鉴定和小鼠胸腺上皮细胞的分离纯化

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

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分离，鉴定和小鼠胸腺上皮细胞的分离纯化

DOI:

10.3791/51780-v

•

07:20 min

•

August 08, 2014

•

Yan Xing, Kristin A. Hogquist

¹Department of Laboratory Medicine & Pathology, Center for Immunology,University of Minnesota

Capítulos

00:05Título
01:24Stromal Cell Isolation from Mouse Thymus
03:20TEC Identification, Panning, and Purification
04:48Results: Representative Thymic Epithelial Populations
06:44Conclusion

Summary

Tadução automática

在这里，我们描述了用于分离，鉴定和小鼠胸腺上皮细胞（TECs的）的纯化的有效方法。该协议可以用于胸腺功能的正常T细胞发育，胸腺机能障碍，和T细胞重建研究。

Tags

Thymic Epithelial Cells Thymus T Cell Development Cell Isolation Cell Purification Cell Characterization Flow Cytometry Cell Depletion Cell Sorting

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