É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 02:11Anesthesia and Surgery
- 03:41Microinjection and DNA Application
- 04:51Electroporation of Testis
- 05:32Wound Closure and Post-surgery
- 06:41Results: In Vivo Microinjection and Electroporation of Mouse Testis
- 07:28Conclusion
This article describes microinjection and electroporation of mouse testis de vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.
