É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:05Lymph Node Preparation and Digestion
- 03:08Staining of Lymph Node Stromal Cells
- 04:13Results: Viability and Surface Marker Expression of Lymph Node Stromal Cells
- 05:17Conclusion
Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.
