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Mus Føtal Hele Tarm Kultur System for<em> Ex vivo</em> Manipulation af signaleringsforløb og Tre-dimensionel Live-Imaging af Villus Udvikling
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Biologia
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JoVE Journal Biologia
Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development

Mus Føtal Hele Tarm Kultur System for<em> Ex vivo</em> Manipulation af signaleringsforløb og Tre-dimensionel Live-Imaging af Villus Udvikling

DOI:
10.3791/51817-v

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06:46 min

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September 04, 2014

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,
1Cell and Developmental Biology,University of Michigan, 2Department of Biosciences and Nutrition,Karolinska Instituet Novum

Capítulos

  • 00:05Título
  • 01:18Whole Organ Culture System
  • 02:55Imaging of Fixed Intestines
  • 04:30Setting Up Live Imaging
  • 05:25Results: Mouse Fetal Whole Intestine Culture System and Imaging
  • 06:20Conclusion

Summary

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Improved imaging technology is allowing three-dimensional imaging of organs during development. Here we describe a whole organ culture system that allows live imaging of the developing villi in the fetal mouse intestine.

Tags

Keywords: Mouse Fetal IntestineEx Vivo Culture3D Live ImagingVillus DevelopmentSignaling PathwaysEpithelium MorphogenesisTwo-photon ImagingPseudostratified Epithelium

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