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Kombination af Microstereolithography og Electrospinning til Fremstil Membraner Udstyret med nicher for Corneal Regeneration
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Kombination af Microstereolithography og Electrospinning til Fremstil Membraner Udstyret med nicher for Corneal Regeneration

DOI:
10.3791/51826-v

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11:42 min

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September 12, 2014

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, , , , , , ,
1Department of Materials Science and Engineering,University of Sheffield, 2Department of Chemistry,University of Sheffield, 3L. V. Prasad Eye Institute

Capítulos

  • 00:05Título
  • 02:15Fabrication of PEGDA Templates by Microstereolithography
  • 03:58Fabrication of Biodegradable PLGA Membranes Using Electrospinning
  • 05:48Long-term Storage of PLGA Microfabricated Membranes
  • 06:34Isolation of Limbal Explants
  • 07:53Inclusion of Ring Scaffolds in Rabbit Cornea 3D Models
  • 08:46Results: Synthetic Microstructured Membranes Support Corneal Regeneration
  • 10:48Conclusion

Summary

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Vi rapporterer en teknik til fremstilling af mikrolommer inden electrospun membraner til at studere celle adfærd. Specifikt beskrives en kombination af microstereolithography og elektrospinning til fremstilling af PLGA (poly (lactid-co-glycolid)) hornhinde biomateriale, som er udstyret med microfeatures.

Tags

Corneal RegenerationMicrostereolithographyElectrospinningCorneal MembranesLimbal Stem CellsPalisades Of VogtCorneal DiseaseCorneal GraftsStem Cell CarriersCorneal Biomaterial DevicesPlatform Technology

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