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动物的基因分型快速其次是建立大脑神经元原代培养
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Neurociência
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JoVE Journal Neurociência
Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
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动物的基因分型快速其次是建立大脑神经元原代培养

DOI:
10.3791/51879-v

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09:51 min

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January 29, 2015

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, , ,
1Department of Molecular Physiology & Biophysics,University of Iowa Carver College of Medicine, 2Department of Psychiatry,University of Iowa Carver College of Medicine, 3EZ BioResearch LLC

Capítulos

  • 00:05Título
  • 01:33Fast PCR Genotyping of Newborn Mice
  • 02:56Primary Culture of Mouse Brain Neurons
  • 08:01Results: Representative Genotyping of Primary Neuronal Culture
  • 09:17Conclusion

Summary

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我们描述了标签和基因分型新生小鼠，并从中产生的主要神经文化的过程。基因分型是快速，有效和可靠的，并且允许进行自动核酸提取。这是为neonatally致死小鼠和其文化，需要事先完成基因分型的特别有用的。

Tags

Keywords: GenotypingPrimary Neuronal CultureRapid GenotypingTattooingLow-density CultureDYT1 DystoniaHippocampusCerebral CortexStriatumNeuronal MorphologyNeuronal Function

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