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Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
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Biologia
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JoVE Journal Biologia
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

DOI:
10.3791/51887-v

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11:53 min

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September 18, 2014

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, ,
1Department of Biochemistry,Université de Montréal, CRCHUM, 2Department of Neurosciences,Université de Montréal, CRCHUM

Capítulos

  • 00:05Título
  • 01:54Isolation of Spinal Cord Mitochondria
  • 05:34Immunolabeling of Isolated Mitochondria for Flow Cytometry
  • 08:06Assaying Mitochondrial Transmembrane Potential and Superoxide Production by Flow Cytometry
  • 09:30Acquisition of Immunolabeled Isolated Mitochondria by Flow Cytometry
  • 10:21Results: Flow Cytometry Analysis of Isolated Mitochondria
  • 11:34Conclusion

Summary

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Described here is a method to detect and quantify mitochondrial outer membrane proteins by immunolabeling of mitochondria isolated from rodent tissue and analysis by flow cytometry. This method can be extended to assess functional aspects of mitochondrial subpopulations.

Tags

Keywords: Mitochondrial Outer Membrane ProteinsFlow CytometryImmunolabelingMitochondrial IsolationSpinal CordMitochondrial PurityHigh-throughput AnalysisMitochondrial SubpopulationsFluorescent Indicator Dyes

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