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Generatie van transgene<em> Hydra</em> Door Embryo Microinjection
JoVE Journal
Biologia
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JoVE Journal Biologia
Generation of Transgenic Hydra by Embryo Microinjection
DOI:

09:10 min

September 11, 2014

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Capítulos

  • 00:05Título
  • 01:21Preparation of Plasmid DNA, Needles, and Injection Dishes
  • 02:56Preparing Embryos for Injection
  • 04:24Microinjection of Plasmid DNA
  • 06:02Culturing and Hatching Embryos
  • 07:19Results: Establishing Transgenic Hydra Lines
  • 08:46Conclusion

Summary

Tadução automática

Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.

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