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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

DOI:
10.3791/51894-v

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06:40 min

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September 09, 2014

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, , ,
1Department of Pathology,Northwestern University

Capítulos

  • 00:05Título
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

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We present an in vitro mouse fetal liver erythroblast culture system that dissects the early and late stages of terminal erythropoiesis. This system facilitates functional analysis of specific genes in different developmental stages.

Tags

Mouse Fetal LiverErythropoiesisErythroid Burst Forming Units (BFU-E)Erythroid Colony Forming Units (CFU-E)Terminal ErythroblastsTER119Erythroid Progenitor CellsGene Function AnalysisEarly And Late Stages Of Terminal ErythropoiesisCDNA OverexpressionShRNA KnockdownErythropoietin (Epo)

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