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Realtidsmålinger membranprotein: Receptor Interactions Brug overfladeplasmonresonans (SPR)
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Biologia
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JoVE Journal Biologia
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Realtidsmålinger membranprotein: Receptor Interactions Brug overfladeplasmonresonans (SPR)

DOI:
10.3791/51937-v

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09:35 min

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November 29, 2014

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, ,
1Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine,The Technion-Israel Institute of Technology

Capítulos

  • 00:05Título
  • 01:25Protein Sample and Buffer Preparation
  • 02:38Sensor Chip and SPR Instrument Setup
  • 03:58Experiment Cycles
  • 07:32Results: On Line Monitoring of Ligand Immobilization and Determination of Kinetic Rate Constants by Injections of Multiple Analyte Concentrations
  • 09:07Conclusion

Summary

Tadução automática

Tadução automática

Overfladeplasmonresonans (SPR) er en etiket-fri fremgangsmåde til påvisning af biomolekylære vekselvirkninger i realtid. Heri en protokol for et membranprotein: er receptorinteraktion eksperiment beskrevet, mens diskutere fordele og ulemper ved teknikken.

Tags

Surface Plasmon ResonanceSPRProtein-protein InteractionsMembrane ProteinsReal-time MeasurementsLabel-freeKineticsAssociationDissociationSensogramABC TransporterSubstrate Binding Protein

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