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De Fluorescence Imaging rapide à moléculaire loi Diffusion sur les membranes cellulaires en direct dans un microscope commercial
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Capítulos
- 00:05Título
- 02:08System Calibration: Point Spread Function (PSF) Calibration
- 05:33Labeled Cell Preparation
- 08:18Data Acquisition Setup
- 10:06Imaging
- 12:39Representative Imaging Results
- 14:39Conclusion
Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.
Tags
Keywords: Fast Fluorescence ImagingMolecular DiffusionLive Cell MembranesCommercial MicroscopeSpatiotemporal ResolutionFluorescently-labeled Plasma-membrane Proteins And LipidsSpatial Autocorrelation FunctionMean Square DisplacementApparent DiffusivityTransferrin ReceptorATTO488 Labeled 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine
