/
/
Évaluation de conformation du VIH-1 trimère glycoprotéines d'enveloppe l'aide d'un base de cellules test ELISA
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:09Polyethylenimine (PEI) Transfection
- 02:55ELISA
- 05:01Results: Detection of HIV-1 Env Expressed at the Surface of Transfected Cells by Monoclonal Antibodies
- 06:52Conclusion
Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.
