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在体外甲基化分析研究蛋白质精氨酸甲基化
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Biologia
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JoVE Journal Biologia
In vitro Methylation Assay to Study Protein Arginine Methylation
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在体外甲基化分析研究蛋白质精氨酸甲基化

DOI:
10.3791/51997-v

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10:01 min

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October 05, 2014

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, , , , , ,
1Department of Pulmonary, Critical Care, Sleep, and Allergy,University of Illinois at Chicago, 2Department of Hematology and Oncology,University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center

Capítulos

  • 00:05Título
  • 01:54Purification of Protein Arginine Methyl Transferases (PRMTs)
  • 05:31Methylation Assay
  • 07:16Confirmation of PRMT Expression and Equal Loading of Substrate
  • 08:38Results: PRMT1 Methylates Ras-GTPase Activating Protein Binding Protein 1 (G3BP1)
  • 09:40Conclusion

Summary

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Tadução automática

蛋白质精氨酸甲基化，由一类酶即催化。蛋白质精氨酸甲基转移酶（PRMTs），是酶促加入甲基组（S），以蛋白质内精氨酸的过程。 在体外甲基化测定法是最可靠的工具，以评估已知的或新型的PRMT底物的甲基化状态。

Tags

Protein Arginine MethylationPRMT1In Vitro Methylation AssayG3BP1S-adenosyl-L-[methyl-3H] MethioninePost-translational ModificationMethyl-arginine Specific AntibodiesProteomic ApproachesBiochemical Assays

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