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创建精确的解剖学和人脑肿瘤的重现性颅内移植瘤

JoVE Journal
Medicina

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JoVE Journal Medicina

Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors

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创建精确的解剖学和人脑肿瘤的重现性颅内移植瘤

DOI:

10.3791/52017-v

•

11:14 min

•

September 24, 2014

•

Angela M. Pierce, Amy K. Keating

¹Department of Pediatrics,University of Colorado School of Medicine

Capítulos

00:05Título
01:32Equipment Preparation
02:32Cell Preparation
03:19Surgical Preparation
04:10Intracranial Injection
08:10Recovery & Tumor Development
08:55Results: Tumor Growth After Intracranial Injection
10:31Conclusion

Summary

Tadução automática

大脑与那些没有得到很好的利用体外或异位的分析代表素质唯一的站点。原位小鼠模型重现的位置及生长特点可以用立体定位仪固定和低压注射泵颅内注射可靠地创建。

Tags

Orthotopic Tumor Models Brain Tumors Intracranial Xenografts Human Brain Tumors In Vivo Models Tumor Cell Engraftment Surgical Technique Reproducibility Tumor Growth Customization

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