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Generatie van geïnduceerde pluripotente stamcellen van spierdystrofie patiënten: Efficiënte-Integratie gratis herprogrammeren van Urine-afgeleide cellen
JoVE Journal
Medicina
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JoVE Journal Medicina
Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells
DOI:

09:11 min

January 28, 2015

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Capítulos

  • 00:05Título
  • 01:46Isolation and Expansion of Urine Cells
  • 04:06Urine Cell Reprogramming Using Sendai Virus
  • 05:30Urine Derived Induced Pluripotent Stem Cell (iPSC) Clonal Expansion
  • 06:53Results: Representative Generation of Viral Genome and Transgene Free iPSCs
  • 08:36Conclusion

Summary

Tadução automática

This protocol entails detailed procedures for isolation of urine derived cells from muscular dystrophy patients; their efficient and rapid reprogramming through Sendai virus transduction.

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