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从开发小脑显微切割分离的不同的细胞群

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection

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从开发小脑显微切割分离的不同的细胞群

DOI:

10.3791/52034-v

•

08:02 min

•

September 21, 2014

•

Larra W. Yuelling, Fang Du, Peng Li, Renata E. Muradimova, Zeng-jie Yang

¹Cancer Biology Program, Fox Chase Cancer Center,Temple University Health System

Capítulos

00:05Título
01:09General Preparations
02:41Dissection and Preparation of Tissue Slices
04:57Tissue Microdissection and Cell Dissociation, Sorting, and Plating
06:46Results of Isolating and Culturing NEPs
07:43Conclusion

Summary

Tadução automática

巢蛋白表达祖细胞是在发展中国家小脑的神经元祖细胞的新发现的种群。用在这里用荧光激活细胞分选组合呈现的显微切割技术中，该细胞群可与任何其他小脑区域污染被纯化和可以培养用于进一步研究。

Tags

Microdissection Nestin-expressing Progenitors (NEPs)Cerebellar External Germinal Layer (EGL)Fluorescent-activated Cell Sorting (FACS)Cell Isolation Cell Culture Percoll Gradient Centrifugation Laser Capture Microdissection

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