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Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
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Biologia
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JoVE Journal Biologia
Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

DOI:
10.3791/52041-v

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10:26 min

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January 16, 2015

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, ,
1Department of Entomology,Virginia Tech

Capítulos

  • 00:05Título
  • 01:22Immature Mosquito Development and Survivorship Assays
  • 03:17Fecundity Assay by Administering Drug Through Artifitial Feeder to Female Mosquitoes
  • 05:56Biochemical Assay of Mosquito Enzyme Inhibition by Drug
  • 08:25Results: Effect of DZNep on Malaria Mosquitoes
  • 10:00Concluision

Summary

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A protocol is developed to examine the effects of an epigenetic drug DZNep on the development, fecundity and survivorship of mosquitoes. Here we describe procedures for the aqueous exposure of DZNep to immature mosquitoes and a blood-based exposure of DZNep to adult mosquitoes in addition to measuring SAH hydrolase inhibition.

Tags

Epigenetic DrugAnopheles GambiaeMalaria VectorToxicological AssayFecunditySurvivorship3-Deazaneplanocin ADZNepS-adenosylhomocysteine HydrolaseInsecticide Resistance

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