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支持的脂双层云母制备的高分辨率光学显微成像
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Bioengenharia
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JoVE Journal Bioengenharia
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
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支持的脂双层云母制备的高分辨率光学显微成像

DOI:
10.3791/52054-v

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07:48 min

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June 07, 2014

•

,
1School of Biological Sciences,Nanyang Technological University

Capítulos

  • 00:01Título
  • 00:47Mica and Slides Preparation
  • 02:26Mica Assembly and Chamber Mounting
  • 05:10Supported Lipid Bilayer (SLB) Formation
  • 05:45Results: Diffusion Analysis of Glass and Mica Supported DOPC Bilayers
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

我们提出了制备云母支持的脂质双层膜为高分辨率显微镜的方法。云母是在原子尺度透明且平坦，但很少采用，因为处理困难成像;我们的制剂导致的云母薄片甚至沉积，并且降低了在双分子层制备中使用的材料。

Tags

Keywords: Supported Lipid BilayersMicaHigh-resolution Optical MicroscopySLB PreparationSLB ImagingLipid Vesicle DepositionCustom-made ChamberAFMSingle-molecule Fluorescence MicroscopyFCS

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