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Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
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Capítulos
- 00:05Título
- 01:27Isolation and Preparation of Primary Bone Macrophages from C57/B16 Mice
- 04:51Co‐incubation of Primary Murine Macrophages and C. neoformans
- 06:56Visualizing Non‐lytic Exocytosis in Real‐time Utilizing Digital Light Microscopy
- 08:11Results: Primary Murine Macrophages Undergo Different Types of Non‐lytic Exocytosis of C. neoformans
- 09:36Conclusion
We describe how to visualize macrophage-C. neoformans (Cn) interactions in real time, with specific emphasis on the process of non-lytic exocytosis using digital light microscopy. Using this technique individually infected macrophages can be studied to ascertain various aspects of this phenomenon.
