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大规模的斑马鱼胚胎的心脏解剖的转录分析
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
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大规模的斑马鱼胚胎的心脏解剖的转录分析

DOI:
10.3791/52087-v

•

10:00 min

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January 12, 2015

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, ,
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology,University of Potsdam, 3Institute of Molecular Biology,Medizinische Hochschule Hannover

Capítulos

  • 00:05Título
  • 01:27Dissecting Zebrafish Embryonic Hearts
  • 04:25Isolating mRNA from Dissected Hearts
  • 07:44Results: Transcriptional Analysis of Dissected Embryonic Zebrafish Hearts
  • 09:38Conclusion

Summary

Tadução automática

Tadução automática

到斑马鱼心脏发育过程中分析的心脏基因表达分布，总RNA已被提取从离体心脏。在这里，我们提出了一个协议，用于收集功能/跳动的心迅速人工清扫，从斑马鱼胚胎获得心脏特异性mRNA。

Tags

Keywords: ZebrafishEmbryonic HeartDissectionTranscriptional AnalysisCardiac TranscriptomeRNA ExtractionQuantitative Real-time PCRFluorescent LabelingFluidic Shear ForceFiltration

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