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在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

JoVE Journal
Biologia

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JoVE Journal Biologia

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

DOI:

10.3791/52104-v

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12:44 min

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November 11, 2014

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Shan Zong*¹, Shuyun Deng*¹, Kenian Chen, Jia Qian Wu

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center,The University of Texas Graduate School of Biomedical Sciences at Houston

Capítulos

00:05Título
02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
03:25EML Cell Culture and Depletion of Lineage Positive Cells
04:52Separation of Lin–CD34⁺ and Lin–CD34^– Cells
07:15RNA Preparation and Library Construction for High-throughput Sequencing
09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
11:38Conclusion

Summary

Tadução automática

RNA测序和生物信息学分析被用来确定显著和差异表达的转录因子在小鼠EMLcells林-CD34 +和林-CD34-细胞亚群。这些转录因子可能在确定自我更新林的CD34 +和偏微分林CD34-细胞间的开关发挥重要作用。

Tags

Keywords: RNA-sequencing EML Cells Hematopoietic Stem Cells Self-renewal Differentiation Transcription Factors Gene Expression Lin-CD34+ Cells Lin-CD34- Cells RNA Isolation Library Construction Data Analysis

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