Journal
/
Imunologia e Infecção
/
西ナイルウイルス変異株の感染に対するMyD88非介在細胞性免疫応答のインビトロ分析
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Please note that all translations are automatically generated. Click here for the English version.

西ナイルウイルス変異株の感染に対するMyD88非介在細胞性免疫応答のインビトロ分析

DOI:
10.3791/52121-v

•

10:39 min

•

November 27, 2014

•

, , , ,
1Department of Microbiology & Immunology,The University of Texas Medical Branch, 2Department of Pathology,The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development,The University of Texas Medical Branch

Capítulos

  • 00:05Título
  • 01:40Flow Cytometry Analysis In Vitro OTII CD4+ T Cell Priming by DC from WNV-infected Mice
  • 05:00Intracellular Cytokine Staining
  • 07:13Results: DC Function and T Cell Responses at an Early Stage of WNV NS4B–P38G Infection
  • 09:45Conclusion

Summary

Tadução automática

Tadução automática

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

Tags

Keywords: West Nile VirusWNVNS4B-P38G MutantMyd88T Cell ResponseDendritic CellsIn Vitro T Cell Priming AssayIntracellular Cytokine StainingCFSEOTII Transgenic MiceMicrocentrifuge Tube SystemBiosafety Level 3

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/52121?article_type=v

アカゲザルの細胞性免疫応答のマルチカラーフローサイトメトリー解析
check_url/pt/52121?article_type=v

細胞星状に7つのステップ
check_url/pt/52121?article_type=v

の寄生蜂の紹介<em>ショウジョウバエ</em>と抗寄生虫免疫応答
check_url/pt/52121?article_type=v

感染を防ぐための新しいアプローチとして、PRP：準備と<em>インビトロ</emPRPの>抗菌特性
check_url/pt/52121?article_type=v

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
check_url/pt/52121?article_type=v

マウスマクロファージにおける先天性免疫応答を調査するために、RNA干渉を使用
check_url/pt/52121?article_type=v

治療薬にリンパ腫細胞の感度での役割を決定するために、好中球の単離と解析
check_url/pt/52121?article_type=v

突然変異体ワクシニアウイルスベクターを構築するためのシンプルで効率的なアプローチ
check_url/pt/52121?article_type=v

イメージング 2 光子生体顕微鏡を用いたインフルエンザ ウイルス感染症の中に気管粘膜の細胞相互作用
check_url/pt/52121?article_type=v

マウスのモデルを評価する生得の免疫反応黄色ブドウ球菌感染症

Read Article