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Imunologia e Infecção
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Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling

DOI:
10.3791/52140-v

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10:03 min

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December 12, 2014

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,
1Inserm U1016,Institut Cochin, 2Cnrs, UMR8104, 3Université Paris Descartes,Sorbonne Paris Cité

Capítulos

  • 00:05Título
  • 01:32Chemokine Stimulation
  • 02:41Cell Staining
  • 04:14Image Acquisition and Analysis
  • 07:23Results: Representative Chemokine-induced T Cell Polarization
  • 09:30Conclusion

Summary

Tadução automática

Tadução automática

Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.

Tags

Cell PolarizationChemokine SignalingImaging Flow CytometryActin CytoskeletonMHC Class IPhospho-ERMPar6/aPKC PathwayPharmacological Inhibition

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