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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
JoVE Journal
Biologia
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JoVE Journal Biologia
Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
DOI:

10:48 min

November 26, 2014

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Capítulos

  • 00:05Título
  • 01:49Reprogramming Adult Human Dermal Fibroblasts with STEMCCA
  • 04:55Vector Integration Site Analysis and STEMCCA Excision
  • 07:08Conversion into GMP-grade Conditions
  • 08:50Results: Reprogramming hiPSCs Using the STEMCCA Lentiviral Approach
  • 09:59Conclusion

Summary

Tadução automática

We describe a protocol for deriving lentiviral-based reprogrammed and characterized factor-free human induced pluripotent stem cells and conversion into putative clinical-grade conditions.

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