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FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis
JoVE Journal
Neurociência
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JoVE Journal Neurociência
FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis

DOI:
10.3791/52207-v

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10:02 min

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December 24, 2014

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, , , ,
1Institute of Neuro and Behavioral Biology,Westfälische Wilhelms-Universität Münster, 2Department of Mathematics and Computer Science,Westfälische Wilhelms-Universität Münster

Capítulos

  • 00:05Título
  • 01:43Crawling Surface Preparation
  • 03:33FIM Imaging Set-up
  • 04:47Fly Handling
  • 05:20FIM Imaging
  • 06:56Tracking of Larval Locomotion
  • 08:14Results: FIM Tracking of Locomotion
  • 09:24Conclusion

Summary

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FIM is a novel, cost effective imaging system designed to track small moving objects such as C. elegans, planaria or Drosophila larvae. The accompanying FIMTrack program is designed to deliver fast and efficient data analysis. Together, these tools allow high-throughput analysis of behavioral traits.

Tags

FIM ImagingFIMtrackLocomotion AnalysisHigh-throughputCost-effectiveNeuronal Network FunctionBehavioral TraitsAutomated QuantificationLocomotion PatternsFrustrated Total Internal ReflectionInfrared IlluminationContour TrackingOpen SourcePlatform IndependentGUICSV FilesResults ViewerDrosophila Larvae

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