/
/
Næringsstof forordning ved Kontinuerlig Fodring for Storstilet Udvidelse af pattedyrsceller in Spheroids
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:05Assemble the Continuous Feeding System
- 04:30Spheroid Formation
- 06:04Continuous Feeding Culture and Adjusted Feed Rate
- 08:50Results: Improved Growth Curve and Cell Yields from Cells Grown in Stirred Suspension Bioreactors
- 10:05Conclusion
Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to intermittent batch feeding for cultures in stirred suspension bioreactors. This study demonstrates the methods required for establishing simple adjusted rate fed cultures.
