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神经元的3-D成像和分析感染<em>在体内</em>与<em>弓形虫</em

JoVE Journal
Neurociência

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JoVE Journal Neurociência

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii

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神经元的3-D成像和分析感染<em>在体内</em>与<em>弓形虫</em

DOI:

10.3791/52237-v

•

06:33 min

•

December 09, 2014

•

Carla M. Cabral, Anita A. Koshy^2,3

¹Bio5 Institute,University of Arizona, ²Department of Neurology,University of Arizona, ³Department of Immunobiology,University of Arizona

Capítulos

00:05Título
01:35Brain Sectioning
02:37Optical Clearing of Brain Tissue Sections
03:56Mounting Brain Tissue Sections
04:59Results: Toxoplasma gondii Cysts Reside within Distant Neuronal Processes
06:00Conclusion

Summary

Tadução automática

使用该协议，我们能够从感染寄生虫弓形虫 ，使可视化和的encysting寄生虫和感染的神经元之间的空间关系分析的小鼠图像160微米厚的脑切片。

Tags

3-D Imaging Neurons Toxoplasma Gondii Intracellular Parasite Brain Infection Central Nervous System (CNS)Neuron Infection Persistent Infection Three-dimensional Visualization Immunostaining Electron Microscopy Serial Sectioning

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