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Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging
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JoVE Journal Medicina
Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

Candida albicans Biofilm Development on Medically-relevant Foreign Bodies in a Mouse Subcutaneous Model Followed by Bioluminescence Imaging

DOI:
10.3791/52239-v

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08:31 min

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January 27, 2015

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, , ,
1Department of Molecular Microbiology, Laboratory of Molecular Cell Biology,Institute of Botany and Microbiology, VIB, KU Leuven, 2Biomedical MRI Unit/ MoSAIC, Department of Imaging & Pathology,KU Leuven

Capítulos

  • 00:05Título
  • 01:51Ex Vivo C. Albicans Adhesion on Fetal Bovinie Serum (FBS)-coated Polyurethane Substrates
  • 03:18Animal Surgery
  • 04:19Bioluminescence Imaging and Analysis
  • 06:26Results: Bioluminescence Signal Measurement
  • 07:30Conclusion

Summary

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Tadução automática

We present an experimental procedure of Candida albicans biofilm development in a mouse subcutaneous model. Fungal biofilms were quantified by determining the number of colony forming units and by a non-invasive bioluminescence imaging, where the amount of light that is produced corresponds with the number of viable cells.

Tags

Keywords: Candida AlbicansBiofilmSubcutaneous ModelBioluminescence ImagingIn VivoForeign BodiesCatheter-associated InfectionsAntifungal Drug Screening

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