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Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
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JoVE Journal Medicina
Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

DOI:
10.3791/52247-v

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06:04 min

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January 23, 2015

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, , , , , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute, 2Lowy Medical Research Institute

Capítulos

  • 00:05Título
  • 01:15RPE Cell Preparation for Injection
  • 02:36Sub-retinal Injection
  • 04:29Results: RPE Subretinal Delivery
  • 05:23Conclusion

Summary

Tadução automática

Tadução automática

Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.

Tags

Keywords: Subretinal InjectionRetinal Pigment EpitheliumRPE CellsStem Cell-derived RPEAnimal ModelAge-related Macular DegenerationPhotoreceptor DegenerationIn Vivo Imaging

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