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細胞イメージングおよびデータ分析：SH-SY5Y神経芽腫細胞における神経伝達物質小胞のダイナミクスを評価するためにTIRFMとpH感受性GFP-プローブ
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Capítulos
- 00:05Título
- 03:32Cell Imaging by Total Internal Reflection Fluorescence Microscopy (TIRFM)
- 06:08Fluorescence Intensity Quantification
- 07:09Photobleaching Correction and Threshold Determination
- 08:16Selection of Fusion Events Using a Semiautomatic Procedure
- 10:01Data Analysis
- 11:31Results: Analysis of Synaptic Vesicles Fusion Under Resting and Stimulated (Potassium-induced Depolarization) Conditions Are Shown
- 12:37Conclusion
This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.
