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A Mouse Fetal Skin Modell av scarless Wound Repair
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JoVE Journal Medicina
A Mouse Fetal Skin Model of Scarless Wound Repair

A Mouse Fetal Skin Modell av scarless Wound Repair

DOI:
10.3791/52297-v

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09:20 min

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January 16, 2015

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, , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Surgery, John A. Burns School of Medicine,University of Hawai’i, 4University of Central Florida College of Medicine

Capítulos

  • 00:05Título
  • 01:07Timed Pregnancies – Superovulation Technique
  • 02:49Murine Fetal Surgery (Dorsal Wounding) on E16.5 and E18.5 Embryos
  • 06:05Post-operative Care and Fetus Harvesting
  • 07:40Results: E16.5 Versus E18.5 Wound Healing
  • 09:00Conclusion

Summary

Tadução automática

Tadução automática

During mammalian development, early gestational skin wounds heal without a scar. Here we detail a reliable and reproducible model of fetal scarless wound healing in the cutaneous dorsum of E16.5 (scarless) and E18.5 (scarring) mouse embryos.

Tags

Fetal SkinWound RepairScarless HealingScar FormationMouse ModelEmbryonic Day 18Lineage TracingIn UteroGenetic ToolsSurgical Model

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