Journal
/
Neurociência
/
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

DOI:
10.3791/52336-v

•

08:29 min

•

February 06, 2015

•

, ,
1Department of Basic Sciences,Mississippi State University College of Veterinary Medicine

Capítulos

  • 00:05Título
  • 01:12Preparaion of Slides and Tissue for Laser Capture Microscopy
  • 03:08Laser Capture Microdissection System
  • 05:19Capturing the Ventral Tegmental Area from PEN Membrane Slides
  • 06:19Results: Dopamine Neuron Isolation Using Laser Capture Microdissection
  • 08:05Conclusion

Summary

Tadução automática

Tadução automática

The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.

Tags

Laser Capture MicrodissectionLCMDopamine NeuronsVentral Tegmental AreaTyrosine HydroxylaseImmunohistochemistryInfrared LaserUV LaserPEN MembraneRNA ExtractionQuantitative PCR

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Laser Capture Microdissection of Drosophila Peripheral Neurons

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Read Article