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बैक्टीरियल भूतल गतिशीलता assays के तैयार करना, इमेजिंग, और मात्रा का ठहराव
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
DOI:

07:35 min

April 07, 2015

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Capítulos

  • 00:05Título
  • 01:44Media and Plate Assay Preparation
  • 03:51Swarm Assay Inoculation
  • 04:51Macroscopic Imaging of Bacterial Surface Motility
  • 05:45Results: Swarming Dynamics of Various Bacteria Strains
  • 06:47Conclusion

Summary

Tadução automática

Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

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