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Forberedelse, Imaging, og Kvantifisering av Bakteriell Surface Motility Analyser
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
DOI:

07:35 min

April 07, 2015

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Capítulos

  • 00:05Título
  • 01:44Media and Plate Assay Preparation
  • 03:51Swarm Assay Inoculation
  • 04:51Macroscopic Imaging of Bacterial Surface Motility
  • 05:45Results: Swarming Dynamics of Various Bacteria Strains
  • 06:47Conclusion

Summary

Tadução automática

Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

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