/
/
مقاربة Optogenetic لتقييم تشكيل الوصلات العصبية في نظام المشارك الثقافة
This content is Free Access.
Capítulos
- 00:05Título
- 02:12Cortical Tissue Dissociation
- 03:52Electroporation and Plating of Primary Cortical Neurons
- 05:14Differentiation of Human Neural Progenitor Cells into Neurons in Co-culture
- 06:55Electrophysiological Recordings of Induced Pluripotent Stem Cell-derived Neurons
- 09:05Results: Co-culture System to Assess Neuronal Connection Formation
- 10:53Conclusion
A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.
