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Imunologia e Infecção
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Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

DOI:
10.3791/52416-v

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10:01 min

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February 11, 2015

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, , , , , , , ,
1Department of Neurosurgery,UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology,UCLA David Geffen School of Medicine, 3Department of Medicine,UCLA David Geffen School of Medicine, 4Brain Research Institute,UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center,UCLA David Geffen School of Medicine

Capítulos

  • 00:05Título
  • 01:58Seeding the Slides with Adherent Tumor Cells
  • 04:05Sedimentation of the Non-adherent T Cells onto the Tumor Cell Monolayer
  • 05:49Data Acquisition, Analysis, and Display
  • 07:41Results: Migration of Alloreactive Cytotoxic T Lymphocytes across Tumor Cell Monolayers
  • 08:55Conclusion

Summary

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We describe a protocol to monitor radial mobility of non-adherent immune cells in vitro using a cell sedimentation manifold/slide apparatus. Cell migration is tracked on monolayers of tumor cells or on extracellular matrix proteins. Examination by light and fluorescence microscopy allows for observation of cell mobility and cytotoxic functionality.

Tags

Keywords: Radial MobilityCytotoxic FunctionRetroviral Replicating VectorNon-adherent Alloresponsive T LymphocytesRadial Monolayer Cell Migration AssayNon-adherent Effector Immune CellsFluorescence MicroscopyCell-to-cell InteractionsCancer Therapy

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