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Boc-अला-अला-ASP-एस-Bzl की hydrolysis: एक वर्णमिति विशेष रूप से granzyme बी proteolytic गतिविधि उपाय है कि परख
JoVE Journal
Química
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JoVE Journal Química
A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
DOI:

05:20 min

November 28, 2014

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Capítulos

  • 00:05Título
  • 01:00Sample Preparation
  • 02:07Granzyme B Activity Assay
  • 03:30Results: The Ala-Ala-Asp-S-Benzyl Substrate is Specific for Granzyme B
  • 04:53Conclusion

Summary

Tadução automática

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

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