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Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway
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Neurociência
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JoVE Journal Neurociência
Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

Direct Visualization of the Murine Dorsal Cochlear Nucleus for Optogenetic Stimulation of the Auditory Pathway

DOI:
10.3791/52426-v

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07:58 min

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January 20, 2015

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, , , , , ,
1Department of Otology and Laryngology,Harvard Medical School, 2Department of Communication Sciences and Disorders,Worcester State University

Capítulos

  • 00:05Título
  • 01:31Primary Craniotomy and Dorsal Cochlear Nucleus Exposure
  • 04:38Pressure Microinjection for Virus-mediated Gene Transfer and Surgical Recovery
  • 05:32Secondary Craniotomy and Cochlear Nucleus Exposure
  • 06:29Results: Secondary Cochlear Nucleus Exposure for Stimulation with Blue Light
  • 07:11Conclusion

Summary

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The goal of this protocol is to outline a surgical approach to provide direct access to the dorsal cochlear nucleus in a murine model.

Tags

Auditory PathwayDorsal Cochlear NucleusOptogenetic StimulationVirus-mediated Gene TransferPosterior Fossa ApproachOpsin InjectionNeural StimulationNeurophysiology Experiments

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