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JoVE Journal
Medicina
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JoVE Journal Medicina
In vitro Enrichment of Ovarian Cancer Tumor-initiating Cells
DOI:

11:37 min

February 18, 2015

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Capítulos

  • 00:05Título
  • 01:43Multicellular Spheroid Generation from Ovarian Cancer Cell Lines
  • 05:02Cell Staining for Flow Cytometric Confirmation of Tumor-initiating Cells (TIC) Markers
  • 07:39Confirmation of Tumorigenicity by In vivo Subcutaneous Injections of TIC Cultures
  • 09:03Results: Low-attachment, Serum-free Conditions Enrich for Cells with TIC Markers and Tumorigenicity
  • 11:06Conclusion

Summary

Tadução automática

Tumor-initiating cells (TICs) may represent a viable therapeutic target for the treatment of ovarian cancer, a highly recurrent and fatal disease. We present a protocol for culture conditions that enrich for this highly tumorigenic population of cells.

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