É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:51Preparation of Mammalian Cells, Viral Production and Transduction for Genetic Barcoding
- 04:59Selection and Amplification of Genetically Barcoded Cells
- 06:28Obtaining Clonal Populations of Genetically Barcoded Cells at Different Intensities
- 08:51Adapt Genetically Barcoded Cell Lines to the Biolgical Application of Choice
- 10:52Results: A Multiplex Analysis of Cleavage of the HIV Envelope and Dengue Virus pr-M Boundary Proteins
- 12:14Conclusion
Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol describes how expression of distinct fluorescent proteins through genetic engineering is used for barcoding individual cells. The procedure enables tracking distinct populations in a cell mixture, which is ideal for multiplexed applications.
