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나의 기능적 특성<sup> +</sup> / H<sup> +</sup> 선택을 양자가 살해 사용하여 세포 내 구획의 교환기는 플라즈마 막에서 그들을 표현하는
JoVE Journal
Biologia
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JoVE Journal Biologia
Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
DOI:

07:38 min

March 30, 2015

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Capítulos

  • 00:05Título
  • 01:28H+ Killing Procedure
  • 02:21Measurement of Intracellular pH
  • 04:16Initial Rate NHE7 Measurement by Fast Li+ Uptake
  • 05:42Results: Representative Selection and Functional Characterization
  • 07:09Conclusion

Summary

Tadução automática

The first part of this article shows how to select mutant cell lines expressing vesicular Na+/H+ exchangers at their plasma membrane. The second part provides protocols based on intracellular pH measurements and fast ion uptake, which are used to determine the ion selectivity and the kinetic parameters of these exchangers.

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