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基于ES-Cell的编程组合MYH6启动子，选择小鼠心脏起搏器细胞聚集一代
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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基于ES-Cell的编程组合MYH6启动子，选择小鼠心脏起搏器细胞聚集一代

DOI:
10.3791/52465-v

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08:52 min

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February 17, 2015

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, ,
1Reference and Translation Center for Cardiac Stem Cell Therapy,University of Rostock

Capítulos

  • 00:05Título
  • 01:19Differentiation Procedure
  • 04:56Selection of Sinus Nodal Cells
  • 07:12Results: Generation of Cardiac Pacemaker Cell Aggregates
  • 08:20Conclusion

Summary

Tadução automática

Tadução automática

本协议描述了如何制作从小鼠多能干细胞（PSC）功能性鼻窦淋巴组织。 T-BOX3（TBX3）表达以及心肌肌球蛋白重链（MYH6）启动抗生素的选择导致了高纯度的起搏细胞聚集。这些“诱导窦房结-机构”（“iSABs”）含有80％以上的起搏细胞，显示高度增加跳动的速度，并能步伐心肌体外 。

Tags

Keywords: Murine Cardiac Pacemaker CellsES-cell-programmingMyh6-promoter-selectionInduced-sinoatrial-bodies (iSABs)Sinus Node Inducer TBX3Pacemaker CellsSpontaneous ContractionNodal Cells

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