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Establecimiento de Human epitelial Enteroids y Colonoids de Whole Tejidos y Biopsia
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
DOI:

06:33 min

March 06, 2015

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Capítulos

  • 00:05Título
  • 01:14Crypt Isolation from Whole Tissue
  • 03:31Crypt Culture in Basement Membrane Matrix
  • 04:27Results: Human Epithelial and Colonoids Cultured from Whole Tissue and Biopsy
  • 05:57Conclusion

Summary

Tadução automática

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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