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斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

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斑马鱼角膜植研究集体细胞迁移和再上皮化在皮肤伤口愈合

DOI:

10.3791/52489-v

•

09:35 min

•

February 25, 2015

•

Jose L. Rapanan, Agnes S. Pascual, Chandana K. Uppalapati, Kimbal E. Cooper, Kathryn J. Leyva, Elizabeth E. Hull

¹Biomedical Sciences Program,Midwestern University, ²Department of Microbiology & Immunology,Midwestern University

Capítulos

00:05Título
01:55Preparation of Anesthesia, Equipment, and Media
02:55Establish Explant Cultures
04:40Brightfield and Video Microscopy
05:20Fixation for Immunofluorescence Microscopy
07:02Results: Cell Migration in Zebrafish Keratocytes
08:40Conclusion

Summary

Tadução automática

斑马鱼角膜基质细胞的表外植迁移，并提供了一个体外模型集体细胞迁移的上皮伤口愈合的情况下机制的研究。这些协议的细节，以建立初级外植体培养在集体细胞迁移测定法使用的有效方式。

Tags

Zebrafish Keratocyte Explant Collective Cell Migration Reepithelialization Cutaneous Wound Healing Single Cell Migration Actin-rich Lamellae Actin Cable Epithelial To Mesenchymal Transition Gene Expression Mutant Transgenic In Vitro Model

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