Journal
/
Medicina
/
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases

A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases

DOI:
10.3791/52495-v

•

09:30 min

•

February 21, 2015

•

, ,
1Department of Psychiatry,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience,Icahn School of Medicine at Mount Sinai

Capítulos

  • 00:05Título
  • 01:22Harvest of Neural Rosettes
  • 03:03Expansion of Neural Progenitor Cells
  • 04:27NPC Transduction
  • 05:15Neurosphere Migration Assay
  • 07:06Results: Patient-specific hiPSCs can be Differentiated into Healthy NPCs and Neurons
  • 08:56Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.

Tags

HiPSCNeural Progenitor CellsNeurological DiseasesPost-mortem StudiesDisease InitiationPatient-derivedDifferentiationNeurospheresGenetic ScreeningCellular Phenotypes

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Utilizing Transcranial Magnetic Stimulation to Study the Human Neuromuscular System

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

Using Saccadometry with Deep Brain Stimulation to Study Normal and Pathological Brain Function

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Setting Up a Stroke Team Algorithm and Conducting Simulation-based Training in the Emergency Department – A Practical Guide

Automated Radiochemical Synthesis of [18F]3F4AP: A Novel PET Tracer for Imaging Demyelinating Diseases

Using Retinal Imaging to Study Dementia

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

Read Article