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交付<em>在体内</em>急性间歇性缺氧新生儿鼠害总理下区源性神经祖细胞培养
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures
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交付<em>在体内</em>急性间歇性缺氧新生儿鼠害总理下区源性神经祖细胞培养

DOI:
10.3791/52527-v

•

05:45 min

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November 02, 2015

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, , ,
1Department of Physical Therapy,University of Florida

Capítulos

  • 00:05Título
  • 01:23Plethysmograph Chamber Set Up and Cycle Time Calibration
  • 02:54Acute Intermittent Hypoxia Administration and Subventricular Zone Stem/Progenitor Cell Culture
  • 04:33Results: Representative Neurosphere Development
  • 05:17Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了管理间歇性缺氧的短期产后1-8天的小鼠或大鼠幼仔的方法。这种方法有效地引起上都在低氧暴露30分钟收获培养的神经祖细胞的一个强大的组织水平“启动效应”。

Tags

In VivoAcute Intermittent HypoxiaNeonatal RodentsSubventricular ZoneNeural Progenitor Cell CulturesCell TherapyNeuronal DifferentiationWhole-body PlethysmographyNon-invasiveNon-stressful

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